Bovine T (Testosterone)ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-OSEL-B0003-24, E-OSEL-B0003-48, E-OSEL-B0003-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: E3

UNIProt ID: ???

Target Synonyms: Testosterone; T;

Research Areas: Signal Transduction, Signal Transduction, Epigenetics and Nuclear Signaling, Developmental Biology

Target Species: Bovine

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Bovine E3 concentrations in serum, plasma.

Tested Sample Types: Serum, Plasma

Type: Competitive

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 0.16 ng/mL

Detection Range: 0.31-20 ng/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine E3 are added to the micro ELISA plate wells. Bovine E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Bovine E3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Bovine E3 in samples. No significant cross-reactivity or interference between Bovine E3 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃, 6 months

Shipping: ice bag