ACE (F940) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3485-50, BS3485-100

Sizes: 50µl, 100µl

Swiss-Prot: P12821

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exo-peptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ACE (F940) polyclonal antibody detects endogenous levels of Angiotensin-converting enzyme protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ACE (F940) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ACE (F940) pAb bin paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 160 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Angiotensin-converting enzyme; Short name=ACE; Dipeptidyl carboxypeptidase I; CD143; ACE1; DCP; DCP1; Angiotensin Converting Enzyme 1

Immunogen: Synthetic peptide, corresponding to amino acids 920-950 of Human ACE.

Conjugate: Unconjugated

Modification: Unmodified